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E.coli HCP LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra E. coli Host Cell Proteins Detection Kit is designed for detection and quantitation of E.coli HCP in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1261C 500 Assay Points 773.00 USD
TRF1261M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra E. coli HCP Detection Kit is designed for detection and quantitation of human E. coli host cell proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Escherichia coli (E. coli) bacteria are commonly utilized as an expression system for the production of human recombinant proteins at industrial scale. However, since very frequently these proteins are intended to be used as therapeutics for humans, they must be subsequently purified to remove undesired contaminants that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target E.coli HCP
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Therapeutic Area Biologics/Bioprocess
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays PDF  1 MB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra E coli HCP TRF1261 PDF  491 KB