PerkinElmer
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DELFIA® Streptavidin-coated yellow plate, 96-well, 10 plates

96-well yellow microplates coated with streptavidin, for biotinylated biomolecules for use in DELFIA time-resolved fluorescence assays.

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AAAND-0005 469.00 USD
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Overview

Streptavidin-coated DELFIA® plates bind all biotinylated biomolecules; e.g. peptides, proteins, antibodies, and haptens. The biotin binding capacity is 10 pmol/well in 200 µL volume. DELFIA Yellow plates have an exceptionally low fluorescence background.

DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Specifications

Assay Technology DELFIA
Automation Compatible Yes
Coating Treatment Streptavidin
Color Yellow
Detection Method Time-Resolved Fluorescence (TRF), DELFIA TRF
Material Polystyrene
Product Brand Name DELFIA
Shipping Condition Ambient
Unit Size Pack of 10
Wells Number 96 well plate

Tools

Resources, Events & More

Resource Type File Name File Format
Application Note Application Notes - Delfia Protein Kinase Assays PDF  461 KB
Application Note DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays PDF  406 KB
Application Note Multiplexing DELFIA® assays using lanthanide-labeled probes PDF  121 KB
Application Note Optimization and Miniaturization of DELFIA TRF Assays Converted from ELISA PDF  2 MB
Application Note Simple Conversion of ELISA to DELFIA TRF Technology PDF  1 MB