PerkinElmer
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DELFIA Sm-Labeling kit (Sm-N1-ITC Chelate), 0.2mg

The DELFIA® Sm-Labeling kit is intended for labeling of proteins with samarium (Sm), for use in multiplexing dissociation enhanced time resolved fluoroimmunoassays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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1244-303
588.00 USD
 
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Overview

Each kit contains 0.2 mg labeling reagent plus Sm-standard, DELFIA Enhancement Solution, Sm-stabilizer, purified BSA for increasing the stability of labeled proteins, an uncoated microtitration strip plate, DELFIA Assay Buffer and DELFIA Wash Concentrate. The kit contents are sufficient for a labeling of up to 1 mg of protein or antibody.

N1 chelates can be used in DELFIA separation assays where the concentration of chelating agents is < 0.05 mmol/L, pH >7, and the temperature is < 38°C. They are suitable for highly sensitive end point measurements in separation based assays such those for detection of protein-protein binding and cell adhesion as well as for immunoassays. Isothiocyanate activated chelates react primarily with free lysines on the compound to be labeled.

Specifications

Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), DELFIA TRF
Experimental Type In vitro
Fluorophore Sm-N1-ITC
Product Brand Name DELFIA
Quantity in a Package Amount 0.02 mg
Shipping Condition Blue Ice
Unit Size 1 Kit
Resources, Events & More
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.

PDF 1 MB

Manual

DELFIA Eu-Labelling kit 1244-302

DELFIA Sm-labeling Reagent is intended for the labeling of proteins with samarium (Sm3+)

PDF 130 KB