PerkinElmer
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DELFIA Eu-N1 Anti-6xHis, 1 mg

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in DELFIA® TRF assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6x histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.

This is a replaced for AD0108.

For research use only. Not for use in diagnostic procedures.

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AD0310
50 μg
505.00 USD
 
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AD0309
1 mg
4715.00 USD
 
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Overview

Generic DELFIA® reagents are intended for use in dissociation-enhanced time-resolved fluorometric assays. All reagents have been labeled using the DELFIA N1-chelate. They are suitable for highly sensitive end point measurements in separation based assays such as those for detection of protein-protein binding and cell adhesion as well as for immunoassays.

DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of auto fluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Specifications

Antibody Conjugates Anti-6X His
Assay Technology Time-Resolved Fluorescence (TRF)
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-N1
Product Brand Name DELFIA
Shipping Condition Dry Ice
Unit Size 1 mg
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Application Note

A Novel DELFIA Time-Resolved Fluorescence Biomarker Detection Assay with Superior Performance to Conventional ELISA

DELFIA® (Dissociated-Enhanced Lanthanide Fluorescent Immunoassay) TRF is a proven, robust immunodetection platform with over 20 years of history. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal, and improved assay dynamic range.

Download this application note to see how DELFIA TRF outperforms ELISA as a robust assay providing high sensitivity, superior signal stability, and a wider dynamic range.

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