384-well clear-bottom, scintillating microplate for cell-based radiometric proximity assays.
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The CytoStar-T scintillating microplate is treated for the growth of tissue culture cells. The microplates are made sterile by gamma-irradiation and treated for adherence of cells by plasma discharge which renders the cell attachment surface hydrophilic. The tissue culture surface is not a coating but rather a stably modified surface with random functional groups covalently bound. The lid has condensation rings that reduce the risk of well-to-well contamination and at the same time reduces evaporation from individual wells, minimizing the potential for "edge effects".
CytoStar-T™ scintillating microplates are designed for non-invasive quantitation in real-time analysis of a wide spectrum of biological reactions in cells under normal physiological conditions. Analyses include cell adhesion, cell signaling (e.g., receptor-ligand binding), cell motility, cell proliferation, normal cellular metabolism, metabolite transport, as well as drug processing (intake and efflux). The technology, which was first developed in 96-well plate format, but is now available in 384-well plate format in response to the increased demand for a higher throughput solution. CytoStar-T scintillating microplates conform to SBS-standards in both the 96-well and 384-well formats. They are sterile, tissue culture treated microplates designed not only for the growth of adherent but also suspension cell cultures (after plate centrifugation or cells settle). The integral, planar, transparent base of each well is composed of a proprietary homogeneous mixture of scintillants and polystyrene. The transparent nature of the base permits the observation of growth of cells plated in the well. Furthermore, radioisotopes having suitable decay characteristics (3H, 14C, 35S, 33P, 45Ca, 125I), brought into proximity with the scintillant contained within the base by virtue of the biological processes within the cells, will have that radioactive decay converted to a light signal. This blue light signal can then be detected and quantified using a PMT-based radiometric detection instrument, like PerkinElmer's MicroBeta2. The amount of light generated is proportional to the amount of radioisotope within, or associated with, the cells.
|Coating Treatment||TC-treated, Sterile, Scintillant|
|Product Brand Name||CytoStar-T|
|Quantity in a Package Amount||5.0 Units|
|Unit Size||5 per case|
|Wells Number||384 well plate|
Cytostar-T scintillating microplates are at the center of the world’s only complete SPA solution. Let PerkinElmer help you choose from among our selection of SPA beads and reagents and scintillation counters to find the right solution for your most challenging cellular problems. From uniquely bright signals to cell visualization to custom coatings, we’ll help you bring more clarity to your research.
We have demonstrated different approaches which can be used for, miniaturization of SPA assays to 384 well microplates. The assay can, be performed at reduced volume, reduced mass or completely reoptimised., The chosen approach will depend on the original 96 well assay performance. The benefits obtained will vary, but in most cases the miniaturized assay will have significant advantages over the 96 well assay with reduced reagent usage and an increase in throughput.
Cell invasion is typically measured using micro-porous filters coated with extra cellular matrix (ECM), which forms a barrier between an upper chamber containing cells and media, and a lower chamber containing media plus chemo-attractant. We have developed a scintillation proximity assay using 96-well Cytostar-T scintillating microplates, to measure the invasion of [14C] and [35S] labeled cells through ECM gel.
Human serum albumin (HSA) is the most important carrier for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner. Several different ligand binding sites have been identified for HSA, two of which are major drug binding sites. Such drug-protein interactions are important in determining drug availability to, and elimination from, the body.
Scintillation Proximity Assay (SPA) technology and LEADseeker homogeneous imaging system are used extensively in high throughput screening. When designing an automated SPA or LEADseeker assay several factors must be taken into consideration, for example: the isotope used, the bead type and liquid handling of beads. Perhaps the major consideration is the method of addition of the beads to the assay. Various different liquid handling devices are available which can carry out this step. Here we review a selection of those evaluated to date for compatibility with SPA.