PerkinElmer
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Bioware® Brite Cell Line HCT116 Red-FLuc

Our bioluminescent light-producing oncology cell lines are labeled with luciferase or dual labeled with luciferase and fluorescent reporters (GFP) for in vivo studies.

Part Number BW124318
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For laboratory use only. This product is intended for animal research only and not for use in humans.

Overview

The HCT116-Red-FLuc Bioware® Brite Cell Line is a light-producing cell line derived from HCT116 human colorectal carcinoma. The cells have been stably transduced with the red-shifted firefly luciferase gene from Luciola Italica (Red-FLuc), for a brighter, red-shifted signal.

  • Organism: Human
  • Tissue: Colon
  • Disease: Colorectal Carcinoma
  • Optical Reporter: Red-FLuc

By emitting intensified, longer wavelength light that is significantly brighter than other firefly luciferases, our bioluminescent oncology cell lines allow you to visualize and monitor the growth of deep tissue tumors in vivo.

After the cells are injected into an appropriate mouse model, the optimized Red-FLuc luciferase enables more sensitive in vivo optical detection with less tissue attenuation so you can detect tumor development earlier, and monitor tumor growth and metastases in both subcutaneous and orthotopic models.

Specifications

Oncology Cell Line Colorectal
Luciferase Classification Firefly
Product Brand Name Bioware Brite
Quantity in a Package Amount 1.0 Units
Shipping Condition Dry Ice
Unit Size 2 million cells
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Application Note

Effects of 5FU and Sorafenib on Proliferation and Biomarker Expression in a Colorectal Cancer Model Using AlphaLISA and EnSight Solutions

A variety of chemotherapeutic drugs with different modes of action have been developed and tested as potential therapies for colorectal cancer. Characterizing the effects of potential drugs with different modes of action is a key part of the process.

In this application note you will learn:

  • How to rapidly measure multiple biomarkers in both cell culture supernatant and lysates from the same wells of a microplate to examine complex protein expression profiles from a colorectal cancer cellular model
  • Benefits of using AlphaLISA® technology for characterizing the effects of potential drugs with different modes of action on a cell culture model of human colorectal cancer
  • How to measure the effects of drug treatments, such as 5FU and Sorafenib, on cellular proliferation by automated well-imaging and cell counting using the EnSight® multimode plate reader
  • Examples of the data and analysis you can generate for such biomarker and proliferation assays

PDF 3 MB

Ebook

In Vivo Imaging Solutions eBook

Researchers trust our in vivo imaging solutions to give them reliable, calibrated data that reveals pathway characterization and therapeutic efficacies for a broad range of indications. Our reagents, instruments, and applications support have helped hundreds of research projects over the years. And our hard-earned expertise makes us a trusted provider of pre-clinical imaging solutions— with more than 9,000 peer reviewed articles as proof.

PDF 4 MB

White Paper

The Role of In Vivo Imaging in Drug Discovery and Development

The primary goal of preclinical imaging is to improve the odds of clinical success and reduce drug discovery and development time and costs. Advances in non-invasive in vivo imaging techniques have raised the use of animal models in drug discovery and development to a new level by enabling quick and efficient drug screening and evaluation. Read this White Paper to learn how preclinical in vivo imaging helps to ensure that smart choices are made by providing Go/No-Go decisions and de-risking drug candidates early on, significantly reducing time to the clinic and lowering costs all while maximizing biological understanding.

PDF 748 KB