ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids. The 1-step format only requires one addition step, and can be used for continuous processing.
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For research use only. Not for use in diagnostic procedures.
ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Our ATPlite 3D and ATPlite 1step 3D luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in 3D cultured mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
ATPlite™ 1Step 3D is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence assay is the alternative to colorimetric, fluorometric, High content microscopic analysis and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured cultured in 3D spheroids. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.
Various cytokines are secreted during an active immune response that can have modulatory effects on target cell populations, including interferon gamma (IFN-ɣ), tumor necrosis factor alpha (TNFa) and several interleukins.
In this application note, you will learn how we investigated:
The effects of secreted factors on a triple-negative breast cancer cell line by treatment with conditioned media from activated PBMCs
Whether the modulatory effects of cytokines secreted by infiltrating immune is different when studied in 3D cell cultures
How to detect and quantify multiple immune-modulated proteins from the same wells in complex cell models grown in 3D spheroid microplates and traditional 2D cell culture using AlphaLISA® technology and EnVision® multimode plate reader in a workflow with ATPlite™ 1step and Opera® Phenix high-content screening system