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AlphaPlex-645 (Sm) Human IL17 Detection Kit, 500 assay points

The AlphaPlex™ 645 (Samarium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
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AP219SM-HV
100 assay points
427.00 USD
 
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AP219SM-C
500 assay points
1810.00 USD
 
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AP219SM-F
5,000 assay points
12000.00 USD
 
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Overview

The AlphaPlex™ 645 (Samarium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

 

AlphaPlex technology allows the detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 645 nm.

 

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

Specifications

Assay Target IL17
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 500 assay points
Resources, Events & More
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.

PDF 1 MB

Application Note

Simultaneous Detection of IL-6 and IL-8 Secretion by Cell Lines using AlphaPlex Technology

This application note describes how AlphaPlex reagents from PerkinElmer can be used to simultaneously detect IL-6 and IL-8 secretion by cell lines.

PDF 437 KB

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