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Anti-FLAG AlphaPlex-545 (Tb) Acceptor beads, 250 µg

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.

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For research use only. Not for use in diagnostic procedures.

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Unit Size
List Price
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AP112TB-C
250 µg
587.00 USD
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AP112TB-M
5 mg
5900.00 USD
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AP112TB-R
25 mg
24100.00 USD
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Detail Information

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-FLAG antibody. The mouse monoclonal M2 clone recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites. These beads can be used to capture FLAG-tagged proteins and peptides, and can be used with Alpha Donor beads to create AlphaPlex no-wash assays for:

  • Protein-protein interactions
  • Protein detection
  • Enzymatic assays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay:

  • 250 µg of Acceptor beads is sufficient to run 250-500 wells using a 50 µL reaction volume
  • 5 mg of Acceptor beads is sufficient to run 5,000-10,000 wells using a 50 µL reaction volume
  • 25 mg of Acceptor beads is sufficient to run 25,000-50,000 wells using a 50 µL reaction volume

Specifications

Antibody Conjugates Anti-FLAG
Automation Compatible Yes
Bead Type or Core Bead Type AlphaPlex Terbium (545) Acceptor
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaPlex
Quantity in a Package Amount 1.0 Units
Shipping Condition Blue Ice
Unit Size 250 µg
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Brochure

Guide

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB