The AlphaLISA® immunoassay kit for human transthyretin enables the quantitative determination of human transthyretin (TTR) in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Transthyretin (TTR) is a transport protein in serum and CSF, most notably know for transporting thyroxine (T4) and retinol binding protein. TTR is produced in the liver where it is secreted into the blood. CSF TTR is produced in the choroid plexus. Misfolding and aggregation of TTR has be linked to amyloid diseases. Drugs have been designed to stabilize TTR and prevent mis aggregation.
|Assay Target Class||Protein|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
This manual describes how to run the AlphaLISA human transthyretin assay kit.