The AlphaLISA® immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor, is a human protein encoded by the gene TNFRSF11B (tumor necrosis factor receptor superfamily member 11B). It is a cytokine decoy receptor for nuclear factor kappa B ligand receptor, and neutralizes its function in osteoclastogenesis. Osteoprotegerin-mediated pathways maintain the structure of healthy bone by inhibiting osteoclast activation. It is released as a disulfide-linked homodimer, though the monomeric form is also present in human serum. The OPG pathway poses a potential therapy for osteopenic disorders, such as osteoporosis, arthritis and hypercalcemia. This AlphaLISA kit will detect both the homodimer and the monomeric forms of osteoprotegerin.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
This manual explains how to run the AlphaLISA no-wash human total osteoprotegerin detection assay.