The AlphaLISA® immunoassay kit for porcine TNFα enables the detection and quantitation of porcine TNFα in porcine serum, plasma, and, cell lysyate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human and bovine TNFα and no cross reactivity towards porcine IFNγ.
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Tumor necrosis factor alpha (TNFα) belongs to the TNF Superfamily and is primarily produced by activated macrophages. The primary role of TNFα is the regulation of immune cells. In porcine, TNFα is a pro-inflammatory cytokine that is involved in a multitude of pathological states such as influenza, coronavirus, tuberculosis, acute viral respiratory diseases, and response to vaccination. This kit is designed to detect TNFα in porcine serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|