The AlphaLISA® immunoassay kit for human TIM-3 enables the quantitative determination of human T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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For research use only. Not for use in diagnostic procedures.
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
TIM-3 (T-cell immunoglobulin and mucin-domain containing-3), also known as HAVCR2 (Hepatitis A virus cellular receptor 2), is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. TIM-3, as an immune checkpoint marker, belongs the famility of inhibitory receptors such as PD-1 and LAG3. TIM-3 is up‑regulated on several populations of activated myeloid cells and T cells (Th1, CD8+, NK, Treg) and plays critical roles in immunosuppressive functions and in anti-tumor immunity. Its binding to Galectin-9 induces a range of immunosuppressive activities which enhance immune tolerance and inhibit anti-tumor immunity. For example, TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. This kit is designated to detect and quantify the levels of TIM-3 in cell culture supernatant, serum, and cell lysates.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|