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Testosterone AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Testosterone Research Kit is designed for detection and quantitation of testosterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
AL324HV 100 assay points 645.00 USD
AL324C 500 assay points 1520.00 USD
AL324F 5,000 assay points 10100.00 USD
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  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

In an AlphaLISA competition assay, a Biotinylated analog of the analyte of interest, the tracer, binds to the Streptavidin-coated Alpha Donor beads, while the Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of low analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. In the presence of high analyte, the beads are separated resulting in lower emission.

Testosterone is the predominant androgenic steroid. This steroid hormone has many functions both in reproductive and non-reproductive tissue. Testosterone plays a key role in the development of testis and prostate. Testosterone levels are also linked with muscle, bone, and body hair growth. Testosterone is observed in most verterbrates, with fish being the largest exception. Testosterone has been used as a treatment for hypogonadism, infertility, osteoporosis, anemia, and low appetite .

Disclaimer:  For research use only. Not for use in diagnostic procedures.


Assay Target Testosterone
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Quantity in a Package Amount 1.0 Units
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  128 KB
Data Sheet Manual AlphaLISA Testosterone Detection Kit AL324 PDF  591 KB
Event Society of Laboratory Automation & Screening (SLAS)