The AlphaLISA® Human Microtubule-associated Protein Tau (TAU) Detection Kit is designed for detection and quantitation of human TAU in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies used in the kit correspond to clone numbers BT2 and Tau 12.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL271C||500 assay points||2827.00 USD|
|AL271F||5,000 assay points||22100.00 USD|
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Microtubule-associated Protein Tau (TAU) is a neuronal protein that binds to axonal microtubules and has roles in the assembly of microtubules, cytoskeletal structure, and axonal transport. Six isoforms of Tau have been described, ranging from 352 to 441 residues, all originating from the alternative splicing of one gene designated MAPT. Tau interacts with tubulin to stabilize the microtubules, but its phosphorylation blocks this association, resulting in disruption of microtubule organization. In humans, hyperphosphorylated Tau can self-assemble into very insoluble aggregates (paired helical filaments) leading to various pathologies. Tauopathy is the name given to diseases associated with Tau accumulation, among which Alzheimer’s disease (AD) is one of the best known. Tau is a biochemical marker for this neurodegenerative disease as an increase of Tau in cerebrospinal fluid is observed in most patients with AD.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Central Nervous System|
|Unit Size||500 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Technical Note||HiBlock Buffer Technical Data Sheet||PDF 333 KB|
|Data Sheet||Manual AlphaLISA Human TAU AL271||PDF 204 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Tau Duplex: A novel assay to detect phosphorylated and total tau protein in neuronal cells using Alpha technology||PDF 515 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|