The AlphaLISA® immunoassay kit for human PSMA enables the quantitative determination of human prostate specific membrane antigen (PSMA) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Prostate specific membrane antigen (PSMA) is an 750 amino-acid type II transmembrane protein. Enzymatic activity for PSMA has been observed, specifically acting as a glutamate-preferring carboxypeptidase. PSMA is expressed in prostate tissues and its expression has been shown to increase with age. Increased expression has been linked with prostate cancer, one of the most common forms of cancer in men. The PSMA AlphaLISA detection kit allows for the detection of PSMA in human sera and cell culture supernatants.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|
This manual explains how to run the AlphaLISA no-wash human prostate specific membrane antigen PSMA