PerkinElmer
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PSMA (human) AlphaLISA Detection Kit, 100 assay points

The AlphaLISA® immunoassay kit for human PSMA enables the quantitative determination of human prostate specific membrane antigen (PSMA) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL3038HV 100 Assay Points 582.00 USD
AL3038C 500 Assay Points 1747.00 USD
AL3038F 5,000 Assay Points 14000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Prostate specific membrane antigen (PSMA) is an 750 amino-acid type II transmembrane protein. Enzymatic activity for PSMA has been observed, specifically acting as a glutamate-preferring carboxypeptidase. PSMA is expressed in prostate tissues and its expression has been shown to increase with age. Increased expression has been linked with prostate cancer, one of the most common forms of cancer in men. The PSMA AlphaLISA detection kit allows for the detection of PSMA in human sera and cell culture supernatants.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target PSMA
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 100 Assay Points

Resources, Events & More

Resource Type File Name File Format
Manual AlphaLISA Human PSMA Detection kit AL3038 PDF  515 KB
Poster Comparison of EMT biomarker expression in 2D monolayer and 3D spheroid cultures in a prostate cancer PDF  1 MB