The AlphaLISA® immunoassay kit for human PON-2 enables the quantitative determination of human Paraoxonase 2 (PON-2) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Paraoxonase 2 (PON-2) is a ubiquitously found bound in membranes. PON-2 works to protect the cell from oxidative stress. PON-2 has been indicated in pathogenic defense response due to its hydrolytic activity against acylhomoserine lactones which plays a key role in bacterial growth. Various vascular diseases are associated with mutations to the PON2 gene.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|