PerkinElmer
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PON-1 (human) AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® immunoassay kit for human paraoxonase/arylesterase 1 (PON-1) enables the quantitative determination of human PON-1 in buffered solution, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL3071HV
100 Assay Points
728.00 USD
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AL3071C
500 Assay Points
1747.00 USD
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AL3071F
5,000 Assay Points
14000.00 USD
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Paraoxonase/arylesterase 1 (PON-1) is a serum enzyme typically only found in mammals. Like most of the paraoxonase family its main function is to act as anti-atherosclerotic component of high-density lipoproteins (HDL). PON-1 levels vary between individuals and has shown to be affected by diet and life-style. PON-1 has shown enzymatic activity against a wide variety of drug classes and toxins including: glucoronides, thiolactones, arylesters, cyclic carbonates, organophosphorous pesticides and nerve gases.

Specifications

Assay Target PON1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Unit Size 500 Assay Points