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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Periostin, also known as osteoblast-specific factor 2, POSTN, or PN, is an extracellular matrix protein that is expressed in wide range of normal adult and fetal tissues, including lung, thyroid, periosteum, placenta, uterus, and GI tract. Periostin binds to integrins which then leads to cell migration and cell adhesion enhancement. High levels of expressed periostin in patients have been associated with cell turnover, cell division, cell invasion, and angiogenesis. It has also been associated with several inflammatory conditions. Periostin plays an important role in the development of teeth and bones but also has been shown to contribute to asthma, atopic dermatitis, and chronic rhinosinusitis.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.