The AlphaLISA® mouse PD-1/PD-L1 binding assay enables development and characterization of small and large molecule anti-mouse PD-1 or PD-L1 reagents for in vitro and in vivo studies, testing of other putative binding ligands such as PD-L2 or characterization of ligand subregions for their ability to block binding, and determination of mouse cross-reactivity of anti-human PD-1 or PD-L1 reagents using a homogeneous AlphaLISA assay (no wash steps).
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Basic immunoregulation studies using human cells can be challenging, whereas options for mouse cells are extensive (immunization of donor mice to generate T cells, T cell receptor transgenic mice). It can often be quicker to learn about immunology and regulation of immune responses by using a mouse system. However, working in mouse systems can often require developing separate mouse reagents if your human drug or antibody won't cross-react with mouse. The AlphaLISA mouse PD-1/PD-L1 binding kit allows determination of cross-reactivity of human anti-PD-1 or PD-L1 reagents with mouse proteins, as well as development or screening of novel mouse PD-1 and PD-L1 reagents that block the binding of this interaction. Using the AlphaLISA mouse PD-1/PD-L1 binding assay, small molecule antagonists or large molecule therapeutics and blocking antibodies can be screened in microplate format. Please note an AlphaLISA human PD-1/PD-L1 binding assay is also available for concurrent studies.
AlphaLISA Binding Assay Features:
AlphaLISA technology allows the detection of binding interactions in a highly sensitive, quantitative, reproducible and user-friendly mode. In the AlphaLISA assay, a biotinylated mouse PD-L1 binds to the Streptavidin-coated Alpha Donor beads, while His-tagged mouse PD-1 is captured by Anti-6X-His AlphaLISA Acceptor beads. When PD-1 binding to PD-L1 occurs, Donor beads and Acceptor beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target||PD-1, PD-L1|
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|
Mouse pharmacological models continue to play a large role in the study of human disease, and mouse tool reagents have shown high utility in immunology and cancer research for decades. It can often be quicker to learn about immunology and the regulation of immune responses using a syngeneic mouse model. However, working in mouse systems can often require the development of separate mouse reagents, if the therapeutic agent of interest does not cross-react with mouse. Find out how the AlphaLISA® human PD-1/PD-L1 and AlphaLISA mouse PD-1/PD-L1 binding assays provide a fast, powerful, homogeneous platform for obtain binding potencies from potential novel drug candidates.