The AlphaLISA® immunoassay kit for human Nicotinamide N-Methyltransferase (NNMT) enables the quantitative determination of human NNMT in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL3079HV||100 Assay Points||728.00 USD|
|AL3079C||500 Assay Points||1747.00 USD|
|AL3079F||5,000 Assay Points||14000.00 USD|
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Nicotinamide N-methyltransferase (NNMT) is a cytosolic enzyme that is typically found in livers, however can still be expressed in other organs. This gene encodes the protein accountable for the enzymatic activity that uses S-adenosyl methionine as the methyl donor. NNMT is known to be a key enzyme in drug metabolism and is related to diseases such as hepatocellular carcinoma (HCC) and Parkinson’s disease. NNMT mRNA levels in patients with HCC are reduced whereas patients’ brains with Parkinson’s disease show high levels of this enzyme.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|