PerkinElmer
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IFNα (mouse) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for IFNα is designed for the quantitative determination of mouse interferon-alpha (IFN-α) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL550HV 100 Assay Points 600.00 USD
AL550C 500 Assay Points 1800.00 USD
AL550F 5000 Assay Points 18000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Inteferon-alpha (IFN-α) is a cytokine involved in innate immune response against viral infection. It is produced by leukocytes and other cell types like plasmacytoid dendritic cells. There are many IFN-α subtypes, with molecular weights ranging from 15 to 21 kDa. They all possess the same antiviral activity, but may differ in their relative biological activities. IFN-α also exhibits several antiproliferative and antitumor activities, making it one of the most used cytokine treatments in cancer patients suffering from hairy cell leukemia, renal carcinoma, Kaposi’s sarcoma, and other malignancies. Under physiological conditions, a low level of IFN-α is detected. However, its production is markedly enhanced during infections and various pathological situations, making IFN-α a major disease marker.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IFNα
Assay Target Class Cytokine
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Mouse
Unit Size 100 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet Manual: AlphaLISA INF-a (Mouse) Detection Kit PDF  524 KB
Event Society of Laboratory Automation & Screening (SLAS)