IL-17A (mouse/rat) AlphaLISA Biotin-free Detection Kit, 5,000 Assay Points

Formats:

• Our HV (high volume) 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 μL reaction volume (10 μL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Interleukin-17A (IL-17A) is the founding member of a group of cytokines called the IL-17 family. The other members are IL-17B, C, D, E, and F. Mature mouse or rat IL-17A is a glycosylated homodimer formed of two 133 amino acid subunits. IL-17A is produced by a subset of T helper cells. It is a proinflammatory cytokine that enhances T cell priming and stimulates macrophages, fibroblasts, endothelial and epithelial cells to produce mediators of inflammation like IL-1, IL-6, and TNFα. It is also critical for neutrophil activation and migration, and induces IL-8, a key chemokine for neutrophils. IL-17 has been involved in the proinflammatory patterns associated with joint inflammation and rheumatoid arthritis in mouse and human models and may be a novel therapeutic target for the treatment of these diseases.

AlphaLISA® technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.