The AlphaLISA® immunoassay kit for human laminin enables the detection and quantitation of human laminin in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-28A, IL-29, and Mouse Laminin.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL368HV||100 assay points||446.00 USD|
|AL368C||500 assay points||1476.00 USD|
|AL368F||5,000 assay points||9700.00 USD|
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Laminin is a high molecular weight (~400KDa) extracellular matrix protein and a major component of basement membrane. It plays an important role in cell differentiation, migration, and adhesion. Laminin has been linked to several health issues including congenital muscular dystrophy, junctional epidermolysis bullosa, and nephrotic syndromes. Furthermore, laminin was observed to be involved in the progression of certain cancers.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Application Note||Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer||PDF 1 MB|
|Application Note||Detection and Modulation of Extracellular Matrix Protein Deregulation in Human Prostate Carcinoma||PDF 1 MB|
|Data Sheet||AlphaLISA Human Laminin AL368||PDF 430 KB|
|Poster||Comparison of EMT biomarker expression in 2D monolayer and 3D spheroid cultures in a prostate cancer||PDF 1 MB|
|Event||Society of Laboratory Automation & Screening (SLAS)|