The AlphaLISA® immunoassay kit for human insulin enables the detection and quantitation of insulin in serum, buffered solution or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The antibodies in the kit are mouse monoclonal antibodies (clone numbers 8E2 and 3A6).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL204C||500 assay points||1476.00 USD|
|AL204F||5,000 assay points||9800.00 USD|
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Insulin is synthesized as a proinsulin hormone of 110 aa by ß-cells of the islets of Langherans of the pancreas. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A of 21 aa and peptide B of 30 aa) that are covalently linked via two disulfide bonds and secreted upon increased glucose concentration in blood. Blood concentration increases from ~50 pmol/L to 300-400 pmol/L 30 min after glucose uptake. Insulin is a key player in the control of both carbohydrate and lipid metabolism and has been implicated in various diseases including diabetes, heart disease and obesity.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Hormone|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Brochure||Alpha product listing||PDF 124 KB|
|Application Note||A Comparison of AlphaLISA and TR-FRET Homogeneous Immunoassays in Serum-Containing Samples||PDF 1 MB|
|Data Sheet||Manual AlphaLISA Human Insulin AL204||PDF 214 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|
|Flyer||AlphaLISA - Frequently Asked Questions||PDF 102 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|