The AlphaLISA® Human Interleukin 5 (IL5) Detection Kit is designed for detection and quantitation of human IL5 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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Human Interleukin 5 (IL5) is produced as a precursor protein maturing into a 115 amino acid protein. The mature active protein is heavily glycosylated and is covalently linked in a homodimer by a disulfide bond, with a native apparent molecular weight of 45-60 kDa. It is produced by Th2 cells, mast cells, and eosinophils. IL5 stimulates B cell growth and promotes the production of cytotoxic T-cells from thymocytes, but its key function is to mediate activation, maturation, and survival of eosinophils. IL5 activated eosinophils eliminate antibody bound parasites through the release of cytotoxic granule proteins. IL5 seems to play a major role in the development of allergic disease/asthma response, which makes IL5 an interesting target for the development of anti-allergy drugs. IL5 is also a potential marker of acute graft-versus-host disease.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.