PerkinElmer
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IL-4 (bovine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL542HV 100 assay points 433.00 USD
AL542C 500 assay points 1433.00 USD
AL542F 5,000 assay points 9500.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cytokines are soluble mediators that impact a multitude of biologies including cell proliferation, survival, death, motility, cell-cell and cell-matrix interactions as well as immune response and leukocyte infiltration. Cytokines are intimately associated with acute and chronic diseases and response to vaccination. Interleukin 4 (IL4) is a cytokine of many biological roles, including stimulation of activated B-cell and T-cell proliferation, differentiation of naive helper T cells (Th0 cells) into Th2 cells, and of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE and up-regulates MHC class II production. Overproduction of IL4 is associated with allergies. IL4, along with other Th2 cytokines, is involved in the airway inflammation with allergic asthma.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL4
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Bovine
Therapeutic Area Inflammation
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits PDF  3 MB
Manual AlphaLISA Bovine IL-4 Kit AL542 PDF  597 KB
Event Society of Laboratory Automation & Screening (SLAS)