The AlphaLISA® immunoassay kit for porcine interleukin 8 (IL-8) enables the quantitative determination of porcine IL-8 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Interleukin-8 (IL-8), also known as CXCL8, is a chemokine produced by monocytes/macrophages, epithelial cells, endothelial cells and neutrophils, in response to inflammatory stimuli. IL8 is a potent chemoattractant for neutrophils, basophils, and T-cells, and causes degranulation of neutrophil specific granules as well as azurophilic granules. In addition, IL8 also has a wide range of other proinflammatory effects. IL8 has been linked to various pathologies, especially respiratory diseases such as pneumonia in porcine.
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|