The AlphaLISA® immunoassay kit for human IL-33 enables the quantitative determination of human interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL3042HV||100 Assay Points||582.00 USD|
|AL3042C||500 Assay Points||1747.00 USD|
|AL3042F||5,000 Assay Points||14000.00 USD|
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Interleukin 33 (IL33), a member of the IL1 family, is a cytokine that drives the production of T helper-2 cytokines. Intercellular molecules such as NF-κB and MAP kinase, are activated by the interaction between IL33 and receptors ST2 and IL1RAP. This pathway drives the production of type-2 cytokines from polarized Th2 cells. Mucosal organs show severe pathological changes when administered with IL33.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
|Resource Type||File Name||File Format|
|Application Note||Utilization of AlphaLISA Technology to Accurately Detect Asthma Biomarkers in Human Serum||PDF 1 MB|
|Manual||AlphaLISA Human IL-33 Detection kit AL3042||PDF 488 KB|