PerkinElmer
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IL-33 (human) AlphaLISA Detection Kit, 100 assay points

The AlphaLISA® immunoassay kit for human IL-33 enables the quantitative determination of human interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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AL3042HV
100 Assay Points
600.00 USD
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AL3042C
500 Assay Points
1800.00 USD
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AL3042F
5,000 Assay Points
14400.00 USD
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Interleukin 33 (IL33), a member of the IL1 family, is a cytokine that drives the production of T helper-2 cytokines. Intercellular molecules such as NF-κB and MAP kinase, are activated by the interaction between IL33 and receptors ST2 and IL1RAP. This pathway drives the production of type-2 cytokines from polarized Th2 cells. Mucosal organs show severe pathological changes when administered with IL33.

Specifications

Assay Target IL33
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 100 Assay Points
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Manual

AlphaLISA Human IL-33 Detection kit AL3042

This manual explains how to run the AlphaLISA no-wash human IL-33 detection assay.

PDF 488 KB