The AlphaLISA® immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL552HV||100 Assay Points||600.00 USD|
|AL552C||500 Assay Points||1800.00 USD|
|AL552F||5,000 Assay Points||18000.00 USD|
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
IGF-1 is a small (70 amino acids) protein with significant resemblance to insulin. However, it is not an analog of insulin in function. It acts as a growth factor during fetal and child development, while acting as an anabolic agent in adults. Abnormal IGF-1 expression can lead to dwarfism, acromegaly, some forms of diabetes and several types of cancer. Some evidence suggests a role in neurological disorders such as ALS. This kit detects rat IGF-1 in serum, plasma and cell culture supernatants.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 128 KB|
|Manual||AlphaLISA Rat IGF-1 Detection kit AL552||PDF 559 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|