PerkinElmer
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IFN-γ (porcine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for porcine IFN-γ enables the detection and quantitation of porcine interferon-gamma in serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human, bovine, ovine, or equine IFNγ. Cross reactivity with other species has not been tested.

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Part Number Unit Size List Price Your Price Quantity
AL549HV 100 assay points 604.00 USD
AL549C 500 assay points 2266.00 USD
AL549F 5,000 assay points 19600.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Porcine IFNγ, the only member of the type II interferon class, is a 20 -25KD homodimer glycosylated cytokine that is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells. It is critical for innate and adaptive immunity against viral, bacterial and protozoan infections, and is involved in tumor growth. In pigs, IFNγ is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Porcine IFNγ has been identified as a biomarker for vaccine responses, viral, and bacterial infection. This kit is designed to detect pIFNγ in serum, plasma, cell culture supernatants, and cell lysates.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IFNγ
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Porcine
Therapeutic Area Inflammation
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits PDF  3 MB
Data Sheet AlphaLISA Porcine IFN-y Detection Kit AL549 PDF  447 KB
Event Society of Laboratory Automation & Screening (SLAS)