The AlphaLISA® Human Interferon β (IFNβ) Detection Kit is designed for detection and quantitation of human IFNβ in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit corresponds to the IFN-ß 1a subtype.
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Mature Interferon-beta (IFN-ß) contains 166 amino acids with a molecular weight of 22 kDa and is produced in large quantities by fibroblasts in response to pathogens. IFN-ß is a cytokine involved in the regulation of unspecific humoral immune responses as well as in immune responses against viral infections. Only small amounts of IFN-ß are produced under healthy conditions. However, levels can dramatically increase during viral infection, inflammation, and autoimmunity. IFN-ß exhibits several biological effects including antiviral and antiproliferative activities. IFN-ß has been used, with some success, as a therapeutic agent in the treatment of multiple sclerosis, as well as in chronic active hepatitis B where it appears to be most promising if the disease has not lasted longer than 5 years.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.