The AlphaLISA® Human Intercellular Adhesion Molecule-1 (ICAM-1) Detection Kit is designed for detection and quantitation of human ICAM-1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies target the extracellular domain of ICAM-1. The analyte in this kit consists of the extracellular domain of human ICAM-1.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Intercellular Adhesion Molecule-1 (ICAM-1) is a dimeric transmembrane glycoprotein with a molecular weight of 80 - 114 kDa. ICAM-1 is a member of the immunoglobulin family and can be expressed on non-hematopoietic cells, such as vascular endothelial cells. It is involved in the transmigration of leucocytes to sites of inflammation. A soluble form of ICAM-1 (sICAM-1) has been identified in serum and cerebrospinal fluid and it contains the five extracellular Ig-domains of ICAM-1. sICAM-1 is an inflammatory marker that has been associated with several common diseases such as diabetes, heart disease, stroke, and malaria. High levels of sICAM-1 have been found in serum from patients with cardiovascular disease, cancer, HIV-1, and autoimmune diseases. sICAM-1 is able to competitively bind the ligands of membrane-bound ICAM-1, such as LFA-1, Mac-1, and human rhinovirus.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.