PerkinElmer
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Utrophin (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human utrophin enables the quantitative determination of utrophin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL3004HV 100 Assay Points 280.00 USD
AL3004C 500 Assay Points 2520.00 USD
AL3004F 5,000 Assay Points 17500.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Utrophin is a muscle protein found at neuromuscular synapse and myotendinous junctions. It is essential for the maintenance of cellular membrane and cytoskeleton structure. Utrophin expression is dramatically increased in patients with muscle weakness and has been involved in Duchenne's muscular dystrophy. In fact, the Utrophin inhibitors are being tested in clinical trials for the prevention of Duchenne's muscular dystrophy. This kit detects Utrophin in serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Utrophin
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 5,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual AlphaLISA Human Utrophin Detection kit AL3004 PDF  423 KB
Event Society of Laboratory Automation & Screening (SLAS)