PerkinElmer
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p62 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for p-62 enables the detection and quantitation of human p62 in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL386HV 100 assay points 557.00 USD
AL386C 500 assay points 1845.00 USD
AL386F 5,000 assay points 12200.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

The ubiquitin binding protein p62 or Sequestosome 1 (SQSTM1) is expressed in most cell types. Synonyms are p62 lck ligand, zeta interacting protein (ZIP, rat), A170 (mouse), OSIL or PDB3. The human protein is 440 amino acids long. It contains an N-terminal protein binding domain (PB1) that interacts with different protein kinases and allows homopolymerization of p62. The domain is followed by several other protein-protein interaction domains and nuclear localization and export signals. Notably, the C-terminal ubiquitin association domain recognizes both mono and polyubiquitin and the LIR motif allows direct binding to the autophagosome-associated protein LC3. p62 plays an important role in the formation of intracellular protein aggregates, autophagy, and NF-B signaling. Mutation of p62 is associated with Paget’s disease (abnormal bone turnover).

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target p62
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Autophagy
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet Manual: AlphaLISA p62 (Human) Detection Kit AL386 PDF  440 KB