The AlphaLISA® immunoassay kit for human COL1A1 enables the detection and quantitation of human COL1A1 in serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL371HV||100 assay points||446.00 USD|
|AL371C||500 assay points||1476.00 USD|
|AL371F||5,000 assay points||9700.00 USD|
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Collagen, type I, alpha 1 (COL1A1) is known as alpha-1 type I collagen that is produced by COL1A1 gene in the connective tissues. Upon synthesis as the pro-alpha1(I) chains, two pro-alpha1(I) chains and one a pro-alpha2(I) chain combine to make up rope-like procollagen molecules that are ultimately converted into Type I collagen by enzymes outside the cells. Type I collagen is well known to maintain and support connective tissues such as cartilage, bone, tendon, and skin. Changes of COL1A1 levels in cells, connective tissues, and in biological fluids are associated with diseases like Ehlers-Danlos syndrome, osteogenesis imperfecta, and osteoporosis.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Collagen|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 128 KB|
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|Data Sheet||AlphaLISA Human COL1A1 AL371||PDF 427 KB|
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|Event||Society of Laboratory Automation & Screening (SLAS)|