The AlphaLISA® Human TGF-β1 Biotin-Free Detection Kit is designed for the quantitative detection of human TGF-β1 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
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The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Transforming growth factor beta 1 or TGF-β1, part of the TGF β cytokine superfamily, is a 25 kD disulfide-linked homodimer. TGF-β1 is produced by many cell types including immune cells and controls cell growth, proliferation, differentiation, and apoptosis by modulating many other cytokines and cytokine receptors. Serum and urine concentrations of TGF β1 are a useful marker for determining the status of patients with diabetic nephropathy in type II.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|