The AlphaLISA® immunoassay kit for human p53 enables the detection and quantitation of human p53 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
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For research use only. Not for use in diagnostic procedures.
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Tumor protein p53, also known as p53, is a well conserved phosphoprotein composed of 390 amino acids. The p53 protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The p53 protein plays a major role in cellular response to DNA damage and other genomic aberrations. The loss of the p53 supressor function through mutations or inactivation of its pathway is associated with most if not all human cancers. Activation of p53 can lead to cell cycle arrest, DNA repair or apoptosis. p53 is phosphorylated at multiple sites by several different protein kinases. This kit quantifies the total amount of p53 in human serum, cell culture supernatants, and cell lysates.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|