The AlphaLISA® Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.
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For research use only. Not for use in diagnostic procedures.
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Interleukin-1 Receptor Antagonist (IL1RA) is a member of the interleukin-1 cytokine family. Immune, epithelial, and adipose cells secrete IL1RA which will bind to the interleukin-1 receptor and inhibit pro-inflammatory signaling through IL-1α and IL-1β. Studies have shown that IL1RA plays a key role in suppressing the pro-inflammatory effects of IL-1 in diseases like rheumatoid arthritis. Additionally, it has been observed that IL1RA may play an important role in diseases like osteoporosis where IL1RA levels are suppressed compared to controls. Furthermore, it has been observed that IL-1 cytokines are abundantly present at tumor sites and the addition of IL1RA can suppress growth of IL-1 producing tumors.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|