PerkinElmer
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hCG (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human chorionic gonadotropin (hCG) enables the quantitative determination of hCG in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL3001HV 100 Assay Points 504.00 USD
AL3001C 500 Assay Points 1512.00 USD
AL3001F 5,000 Assay Points 15100.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone typically detected in pregnancy tests but can also be produced by some cancerous tumors. It is heterodimeric in which the alpha subunit is shared amongst other similar glycoproteins. The beta subunit is unique to hCG. hCG interacts with the LHCG receptor in order to promote maintenance of the corpeus luteum at the beginning stages of pregnancy. hCG is produced by the syncytiotrophoblast in the placenta. The beta subunit is excreted by various cancers such as seminoma and germ cell tumors.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target hCG
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual AlphaLISA hCG Detection kit AL3001 PDF  676 KB
Event Society of Laboratory Automation & Screening (SLAS)