PerkinElmer
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ApoB (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human ApoB enables the quantitative determination of human apolipoprotein B in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL392HV 100 Assay Points 582.00 USD
AL392C 500 Assay Points 1747.00 USD
AL392F 5,000 Assay Points 17500.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Apolipoprotein B (ApoB) is a major protein component of Low, Very low, and Intermediate Density Lipoproteins. Although the exact function of ApoB remains unclear it is apparent that ApoB plays a major role in organization and formation of these lipoprotein complexes. ApoB is currently used as a biomarker to predict cardiovascular diseases, where higher levels have been associated with an increased risk. This AlphaLISA detection kit allows for the detection of ApoB levels in serum, plasma, and cell culture supernatants.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target ApoB
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual AlphaLISA Human ApoB Detection kit AL392 PDF  418 KB
Event Society of Laboratory Automation & Screening (SLAS)