PerkinElmer
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Alpha-1 Anti-Trypsin (A1AT) (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human A1AT Detection Kit is designed for detection and quantitation of human Alpha-1 Anti-Trypsin (A1AT) in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL344HV
100 assay points
653.00 USD
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AL344C
500 assay points
1538.00 USD
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AL344F
5,000 assay points
10200.00 USD
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Alpha-1 Antitrypsin (A1AT) is a 52 KD protein. The serpin superfamily includes the protease inhibitors alpha-1 antitrypsin and antithrombin, which are widely studied due to their involvement in the blood coagulation and inflammation. A deficiency in antitrypsin causes emphysema ((Chronic obstructive pulmonary disease (COPD), also known as chronic obstructive lung disease (COLD), and chronic obstructive airway disease (COAD)). Circulating levels of A1AT is an excellent indicator of patients with inflammatory diseases. The present kit detects hA1AT in human serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target A1AT
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 500 assay points
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Application Note

Measuring PD-L1 Expression in Breast Cancer Cell Lines with AlphaLISA

Too many candidates, too little time. The lack of robust, rapid, high-throughput assays to identify and qualify potential therapeutic targets in areas such as cancer research continues to cost valuable time. What if you could increase assay throughput without compromising sensitivity, obtain more data points from each sample and eliminate tedious wash steps? Find out how AlphaLISA® assay technology, combined with the EnVision® multimode plate reader, provides a fast, powerful, homogeneous platform for screening potential inhibitors of PD-L1 (a protein associated with breast cancer tumor cells) expression in human cells.

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