The AlphaLISA® immunoassay kit for human GITR enables the quantitative determination of human glucocorticoid-induced TNFR-related protein (GITR) in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Glucocorticoid-induced TNF-related Protein (GITR), also known as TNFSF18, is a transmembrane receptor protein expressed on peripheral blood T cells and in bone marrow. It shares homology with 4-1BB and CD27. Ligation of GITR activates NfκB and protects from TCR activated cell death. Activation of GITR by anti-GITR antibodies has been shown to be effective in inducing antitumor immune response.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
Protocol for AlphaLISA human GITR Detection Kit