The AlphaLISA® immunoassay kit for human FASL enables the quantitative determination of human FAS ligand in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
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For research use only. Not for use in diagnostic procedures.
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
FAS Ligand (FASL), also known as CD95L, is a transmembrane protein expressed on cytotoxic T cells. It binds to CD95 regulatory T-cells. Upon binding to its receptor, the protein forms trimers that initiate apoptosis of the cells. The protein is involved in regulation of cytotoxic T-cells levels, protection of self-immune effects and control of inflammation. However, some cancer cells can overexpress FASL, blocking the immune response from destroying the tumor.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|