Collagen IV (human) AlphaLISA Detection Kit, 100 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Collagen IV, as one of many extracellular matrix proteins, is produced by fibroblasts, endothelial cells, and epithelial cells. It is a heterotrimeric molecule containing two α1-like and one α2-like chain. The chain compositions of collagen IV are associated with six genes (COL4A1, COL4A2, COL4A3, COL4A4, COL4A5, COL4A6) suggesting that the exisistence of additional alpha-chains to collagen structures and functions. Collagen Type IV is found mainly in the basement membranes of many tissiues and organs including liver, kindney, and skin. It functions to maintian the structural integrity of cells, tissues, and organs. Structural alterations of collagen IV is associated with many diseases such as nephritic syndrome, hemoptysis, liver fibrosis, subepidermal blistering diseases in the skin, and diabetic nephropathy. Concentrations of collagen IV in serum correlate with alcoholic liver disease.