PerkinElmer
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CD40 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human CD40 enables the quantitative determination of human cluster of differentiation 40 protein in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
AL3102HV
100 Assay Points
782.00 USD
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AL3102C
500 Assay Points
2213.00 USD
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AL3102F
5,000 Assay Points
11100.00 USD
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Cluster of differentiation 40 (CD40), also known as TNFRSF5, is a type II transmembrane receptor protein expressed on B cells, dendritic cells, activated monocytes, and others. It binds to CD40L. Ligation of CD40 results in the aggregation of CD40, which in turn initiates signaling pathways and stimulates B-cell proliferation. CD40 expression has been shown to be upregulated in a wide range of tumor cells including Non-Hodgkin’s and Hodgkin’s lymphomas.

Specifications

Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 5,000 Assay Points