PerkinElmer
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CCL5/RANTES (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) Detection Kit is designed for detection and quantitation of human CCL5/RANTES in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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AL289C
500 assay points
2912.00 USD
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AL289F
5,000 assay points
22800.00 USD
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Detail Information

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

C-C Motif Chemokine 5 (CCL5), also known as Regulated upon Activation, Normal T cell Expressed and Secreted (RANTES), is a 7.8 kDa protein that plays a primary role in the inflammatory immune response. CCL5 binds to C-C chemokine receptor (CCR) types 1, 3, and 5. CCL5 dimerization and oligomerization, as well as binding to glycosaminoglycan, are key elements of cell recruitment in the inflammation process. CCL5 is a potent chemoattractant for a number of different cell types, particularly eosinophils, basophils, and mononuclear cells. CCL5 is a potential biomarker for several diseases, for example, it can be useful for indicating the magnitude of asthmatic airway inflammation. In addition to being an important biomarker, CCL5 is studied for its interaction with its receptor, CCR5, in human immunodeficiency virus (HIV) research.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target CCL5
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 500 assay points
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Brochure

Poster

A Comparison of AlphaLISABead-Based Luminescence and Electrochemiluminescence Immunoassay Technologies for Detection of Human EPO, Amyloid Beta 42 and VEGF in Complex Sample Matrices

For the detection of three biomarkers in complex sample matrices, the AlphaLISA and Electrochemiluminescent (ECL) assay technologies were shown to have similar: Assay windows (linear dynamic range), Lower and upper detection limit, Intra-and inter-assay precision (lower % CV) The advantages of using AlphaLISA over ECL are: Shorter total assay duration No wash steps No shaking Lower sample volume requirement for equivalent performance Less expensive instrument and plates required

PDF 179 KB
AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices

The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.

PDF 273 KB