PerkinElmer
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IgM (bovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgM in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL533HV
100 assay points
645.00 USD
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AL533C
500 assay points
2060.00 USD
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AL533F
5,000 assay points
10300.00 USD
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Detail Information

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. Bovine immunoglobulin M (bIgM) is the largest antibody isotype (900kDa ) and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen-B-cells. Due to this large size, IgM is typically isolated to the serum. Bovine IgM whole molecule is ideal for investigators in Immunology, Microbiology, and Cell Biology. Typically, bovine serum and plasma samples contain about 0.6 to 4.3 mg/mL of bIgM. The present kit permits detection of bovine IgM (i.e. analyte) in bovine serum and plasma.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target IgM
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Bovine
Therapeutic Area Biologics/Bioprocess
Unit Size 100 assay points