The AlphaLISA immunoassay kit for detection and quantitation of bovine IFNγ in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL535HV||100 assay points||645.00 USD|
|AL535C||500 assay points||2060.00 USD|
|AL535F||5,000 assay points||10300.00 USD|
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IFNγ is mainly produced by activated T lymphocytes. Although by itself it has limited direct inflammatory effects, IFNγ is a major macrophage-activating factor, increasing TNFα and IL-1α production and thus contributing to activate acquired immunity to disease and passive immunity to vaccination. In particular, IFNγ has been associated to immune responses against bovine tuberculosis caused by Mycoplasma bovis. The present kit permits detection of bovine IFNγ (i.e. analyte) in bovine serum, plasma, and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Application Note||Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits||PDF 3 MB|
|Data Sheet||Manual AlphaLISA Bovine IFNγ AL535||PDF 224 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|